Microfluidic chip - based Multiple - Locus Variable - Number Tandem Repeat 1 Fingerprinting ( MLVF ) with new primer sets for Methicillin - Resistant Staphylococcus
نویسندگان
چکیده
Microfluidic chip-based Multiple-Locus Variable-Number Tandem Repeat 1 Fingerprinting (MLVF) with new primer sets for Methicillin-Resistant Staphylococcus 2 aureus. 3 4 Artur J. Sabat, Monika A. Chlebowicz, Hajo Grundmann, Jan P. Arends, Greetje 5 Kampinga, Nico E. L. Meessen, Alexander W. Friedrich* and Jan Maarten van Dijl 6 7 Running title: MLVF on microfluidic chips for subtyping MRSA isolates 8 9 Department of Medical Microbiology, University of Groningen, University Medical Center 10 Groningen, The Netherlands. 11 12
منابع مشابه
Use of the multiple-locus variable-number tandem repeat fingerprinting method and microfluidic chips for rapid genotyping of Staphylococcus aureus isolates
Introduction / objectives The early detection of outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) and a rapid and accurate identification of sources and routes of transmission are important objectives in hospital settings. In this study we investigated the application potential of Multiple-Locus Variable number tandem repeat Fingerprinting (MLVF) combined with microfluidic techno...
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Methicillin resistant Staphylococcus aureus (MRSA), particularly strains with type III staphylococcal cassette chromosome mec (SCCmec), represent a serious human pathogen in Tehran, Iran. The disease-causing capability depends on their ability to produce a wide variety of virulent factors. The prevalence of exotoxin genes and multiple-locus variable number of tandem repeats fingerprinting (MLVF...
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BACKGROUND Multiple-locus variable-number tandem-repeat fingerprinting (MLVF) is based on multiplex PCR, utilizing variable number tandem repeat. Our goal was to compare the performance of MLVF in distinguishing clinical Staphylococcus aureus isolates with that of pulsed-field gel electrophoresis (PFGE), which has traditionally been the gold standard. METHODS Sixty-three clinically significan...
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Methicillin-resistant Staphylococcus aureus (MRSA) represent a serious threat for public health worldwide. Of particular concern is the emergence of community-acquired MRSA, which is often difficult to distinguish from nosocomial MRSA due to a lack of suitable typing methods for early detection. For example, the USA300 pulsed-field gel electrophoresis (PFGE) pattern includes both the 'classical...
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Methicillin-resistant Staphylococcus aureus (MRSA) has become an important nosocomial pathogen, causing considerable morbidity and mortality. During the last 20 years, a variety of genotyping methods have been introduced for screening the prevalence of MRSA. In this study, we developed and evaluated an improved approach capillary gel electrophoresis based multilocus variable-number tandem-repea...
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